How should a biopsy sample be stored?

Article by: Ing. María Arriaga Jr. | Last update: April 10, 2022
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Inform the laboratory immediately after obtaining the sample, in order to speed up collection. Keep all samples in a refrigerator (2-8ºC) until collection. Plasma samples that cannot be sent within 24 hours must be frozen at – 20°C

How are biological samples stored?

Refrigeration of biological samples is a good solution for short-term storage of samples that are stable at temperatures between 4 and 8ºC. They can be stored refrigerated: Biological reagents such as enzymes or antibodies that are used frequently.

How to send biopsy sample?

Submission of biopsies (punch or surgical type) or organs (whole or pieces) These types of samples should ALWAYS be sent in 10% formalin (1 part 40% commercial formaldehyde to 9 parts tap water). We then passed it to a buffered formalin.

How long can a sample remain in formalin?

It is recommended that they be used: for a surgical piece, between 24 and 48 hours of fixation; for a needle biopsy, more than 6 hours; and, in general, a fixation time that does not exceed 72 hours.

How long should surgical and cytological samples be kept in the files of the Pathology Laboratory?

2- It is recommended that excess tissue samples, from both Surgical Pathology and Clinical Autopsies, fixed in formaldehyde and not included in paraffin, be kept for a minimum of 30 days from the date of validation of the corresponding report.

21 related questions found

How to preserve a sample for pathology?


Inform the laboratory immediately after obtaining the sample, in order to speed up collection. Keep all samples in a refrigerator (2-8ºC) until collection. Plasma samples that cannot be sent within 24 hours must be frozen at – 20°C

How long do paraffin sections keep?

5.7.1 The minimum time that the archived material, such as slides and paraffin blocks, must be kept in the establishments or laboratories, will be no less than three years.

How to preserve an organ in formalin?

10% formalin is used to preserve earthworms, snails, small vertebrate animals and soft tissue organs (heart, kidney, others). The bottles are kept in a cool place, away from heat sources to avoid fire risks. They must be properly labeled.

How should formalin be stored?

Storage conditions: Formalin should be stored at room temperature (approximately 25°C). Under such conditions it remains stable for 6 months. Stored at cooler temperatures, stability drops considerably.

How long can a cytology sample be kept?

Specimens can be stored and transported for up to 2 weeks at room temperature. Transport to the analysis laboratory does NOT require refrigeration. Depending on the test chosen, they can be stored in the laboratory for up to 1 week at 4°C and up to 3 months at -20°C.

How to send a sample to the laboratory?

Collect the sample in a sterile, airtight bottle. It will be shipped refrigerated, never frozen. Whole blood It will be sent in hermetic tubes with anticoagulant, in refrigeration and in the first 24 hours after the extraction. Send in airtight tubes without anticoagulant, perfectly identified and refrigerated.

How should laboratory samples be transported?

To do this, the sample is packed in an airtight container, well covered, which is placed inside a second unbreakable airtight container. An absorbent material must be placed between both containers to retain the content in case of breakage.

How are the samples stored when they arrive at the laboratory?

Shipping time and conservation. The sample must be sent to the laboratory within 1 hour, stored at room temperature (2). If this is not possible, store in a refrigerator at 2-8 ºC for a maximum of 24 h (3). (1) The sample must be free of fecal contamination or toilet paper.

What is sample storage?

Process and result of storing a soil sample under predefined conditions for a normally specified time interval between taking and subsequent treatment of the sample.

What happens if I mix formaldehyde with water?

Formaldehyde dissolves in water (400 ml gas/L water at 20 °C). The solution slowly degrades to form paraformaldehyde, the polymer of formaldehyde. The cyclic trimer can also be formed. The oxidation of formaldehyde produces formic acid and, in a second stage, water and carbon dioxide.

How is formalin neutralized?

D-Formalizer is a safe chemical treatment to neutralize formaldehyde residues. Leaves a clear solution, free of solids and aldehyde odour, which can be disposed of without clogging drains. Simply add one packet of D-Formalizer to 4 Liters of used formalin (or 4% glutaraldehyde) and shake gently.

How to handle formaldehyde?

How to handle the product

It should be mixed in places outdoors or with a lot of ventilation. Some precautions must be taken and both a mask and gloves must be worn for our protection. The product should not be used directly on the skin. It should be out of reach of children or pets.

How long is an organ preserved in formalin?

Fixation. The piece is immersed in 5% formalin for approximately 15 days until total tissue fixation is achieved.

How long does an organ last in formalin?

Organs exposed to normothermic ischemia remain viable for relatively short periods, usually less than 1 hour. However, by cooling the organ from 37°C to 2–4°C, the preservation time can be extended by a period of 12–13 h5.

How is an organ preserved?

The most common method of preservation, and also the least expensive, is to perfuse or wash the organ internally with a cold preservation solution immediately after extraction in the operating room itself.

How is the section of frozen tissue performed?

The frozen tissue block (with Isopentane at -70º, or liquid nitrogen), is cut with a device called Cryostat (A cryostat is a microtome mounted inside a freezer for processing frozen tissue). The paraffin-embedded tissue is subjected to very fine sections with a so-called microtome.

What is a frozen cut?

The one that is performed with a microtome, for which it is previously necessary to harden the tissues that are going to be sectioned.

When is the paraffin technique used?

The paraffin embedding technique is a perfusion of the same in the tissues to create a homogeneous medium, which allows making thinner sections with great ease and greater precision than when they are made by freezing.

How long does a pathology sample last?

These procedures are usually quite quick and can take 15 to 30 minutes to perform, depending on the part of the body being biopsied. Usually, the biopsy sample is then stored in a special type of preservative and sent to the pathology lab for processing.

What is a pathological sample?

In pathological anatomy, the samples par excellence are the histological preparations obtained from a problem tissue using microtomes.

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